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MedChemExpress 13757a
13757a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tamoxifen  (MedChemExpress)
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MedChemExpress tamoxifen
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Tamoxifen, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/pmc13457617-435-0-1
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mi surgery  (MedChemExpress)
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MedChemExpress mi surgery
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Mi Surgery, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/pm42521400-60-3-15
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transgenic tmem119 creer t2  (MedChemExpress)
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MedChemExpress transgenic tmem119 creer t2
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Transgenic Tmem119 Creer T2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/pmc13383698-248-1-10
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cycloheximide chx medchemexpress cat  (MedChemExpress)
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MedChemExpress cycloheximide chx medchemexpress cat
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Cycloheximide Chx Medchemexpress Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/pm42441400-294-59-61
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adult mice  (MedChemExpress)
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MedChemExpress adult mice
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Adult Mice, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/bio_rxiv__64898__2026__07__02__736225-199-6-18
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cre activation tamoxifen  (MedChemExpress)
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MedChemExpress cre activation tamoxifen
2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of <t>Tamoxifen,</t> red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)
Cre Activation Tamoxifen, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tamoxifen/Tamoxifen/pm42358011-40-8-10
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2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of Tamoxifen, red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)

Journal: Cell Regeneration

Article Title: Small-molecule cocktail 2SM promotes heart regeneration via inducing embryonic-like cardiomyocyte state

doi: 10.1186/s13619-026-00301-0

Figure Lengend Snippet: 2SM promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction (MI) in vivo. A Schematic of 2SM delivery and heart harvest in adult αMHC-MerCreMer; MADM mice after MI. Blue arrows represent the administration of Tamoxifen, red arrows represent the administration of vehicle or 2SM. B Representative images and quantification of single-colored cardiomyocytes in αMHC-MerCreMer; MADM mice at 7 day post injury (dpi) after administration of vehicle or 2SM. Scale bar, 20 μm. Vehicle: n = 9 animals; 2SM: n = 9 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (* P < 0.05). C Schematic of 2SM delivery, echocardiography and heart harvest in adult rats after MI. Red arrows represent the administration of vehicle or 2SM, the time points at which echocardiography was performed to assess cardiac function . D and E Representative images and quantification of KI67-positive (D) and pH3-positive ( E ) cardiomyocytes in the border region at 7 dpi. Arrowheads indicate positive cardiomyocytes. Cardiomyocytes were identified by TNNT2 and ACTN2 (α-Actinin) staining (red). Scale bar, 20 μm. Vehicle: n = 10 animals; 2SM: n = 10 animals; analyzed from 3 sections per animal. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (**** P < 0.0001). F and G Serial echocardiographic assessment of ejection fraction (EF%, F) and fractional shortening (FS%, G) from vehicle or 2SM-treated rats at multiple time points after MI. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant; ** P < 0.01, **** P < 0.0001). H Representative Masson's trichrome staining images from serial cross-sections (from base to apex) of rat hearts treated with vehicle or 2SM at 56 dpi, and quantification of infarct size. Scale bar, 5 mm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student's t -test (*** P < 0.001). I Representative images and quantification of cardiomyocyte cross-sectional area measured by wheat germ agglutinin (WGA) staining at 56 dpi. Scale bar, 50 µm. Vehicle: n = 12 animals; 2SM: n = 12 animals. Data represent mean ± SEM, with individual animals shown as dots overlaid on the bar graphs. Statistical analysis by Student’s t -test (ns, not significant)

Article Snippet: Tamoxifen (MCE, HY-13757A) was dissolved in corn oil (MCE, HY-Y1888) at a concentration of 20 mg/mL by shaking overnight at 37 °C.

Techniques: In Vivo, Staining